Journal: Antioxidants

Article Title: Intrarenal Arterial Transplantation of Dexmedetomidine Preconditioning Adipose Stem-Cell-Derived Microvesicles Confers Further Therapeutic Potential to Attenuate Renal Ischemia/Reperfusion Injury through miR-122-5p/Erythropoietin/Apoptosis Axis

doi: 10.3390/antiox11091702

Figure Lengend Snippet: Effect of MVs or DEXMVs on pathologic parameters in renal cortex and medulla by H&E stain ( A ), pNF-κB, Caspase-3, and PARP stain ( B ) in CON, IR, MV, and DEXMV groups. The statistical data of tubular injury score ( C ), p-NF-κB expression ( D ), Caspase-3 expression, ( E ) and PARP expression ( F ) are indicated. Data are expressed as mean ± SEM in each group (n = 6) using single values. * p < 0.05 compared with CON group. td: tubular dilation. Yellow arrows in ( A ) indicate tubular dilation (td). Asterisks in ( A ) indicate erythrocyte accumulation. Yellows in ( B ) indicate each brown stain. MV, microvesicle; IR, ischemia/reperfusion; IRMV, ischemia/reperfusion with microvesicle; IRDEXMR, ischemia/reperfusion with dexmedetomidine preconditioned microvesicle; PARP, poly-(ADP-ribose)-polymerase; SEM, standard error mean. # p < 0.05 compared with IR group. a p < 0.05 vs. MV group.

Article Snippet: Antibodies raised against the monoclonal mouse anti-human PARP (Promega, Madison, WI, USA), polyclonal rabbit anti-rat caspase 3 (Chemicon, CA, USA), primary antibody Bcl-2 (Santa Cruz, Dallas, TX, USA), Bax (Abcam, Cambridge, UK), rabbit polyclonal EPO (Abcam, Cambridge, UK), and monoclonal mouse anti-mouse β-actin (Sigma, Saint Louis, MI, USA) were used at 1:400.

Techniques: Staining, Expressing

Journal: Antioxidants

Article Title: Intrarenal Arterial Transplantation of Dexmedetomidine Preconditioning Adipose Stem-Cell-Derived Microvesicles Confers Further Therapeutic Potential to Attenuate Renal Ischemia/Reperfusion Injury through miR-122-5p/Erythropoietin/Apoptosis Axis

doi: 10.3390/antiox11091702

Figure Lengend Snippet: Effect of ADSC-derived MVs (IRMV) or DEX preconditioning ADSC-derived MVs (IRDEXMV) on Bax, Bcl-2, cleaved caspase-3 (c-caspase-3), PARP, and EPO expression in the four groups of kidneys. ( A ): The original data of Western blot. ( B ): Statistical data of the ratio of Bax/Bcl-2. ( C ): Statistical data of the ratio of C-Casp 3/actin. ( D ): Statistical data of the ratio of PARP/actin. ( E ): Statistical data of the ratio of EPO/actin. Data are expressed as mean ± SEM in each group (n = 3) using the single values. * p < 0.05 compared with CON group. ADSC, adipose stem cells; c-caspase-3, cleaved caspase-3; EPO, erythropoietin; MV, microvesicle; IR, ischemia/reperfusion; IRMV, ischemia/reperfusion with microvesicle; IRDEXMR, ischemia/reperfusion with dexmedetomidine preconditioned microvesicle; PARP, poly-(ADP-ribose)-polymerase; SEM: standard error mean. # p < 0.05 compared with IR group. a p < 0.05 compared with IR+MV group.

Article Snippet: Antibodies raised against the monoclonal mouse anti-human PARP (Promega, Madison, WI, USA), polyclonal rabbit anti-rat caspase 3 (Chemicon, CA, USA), primary antibody Bcl-2 (Santa Cruz, Dallas, TX, USA), Bax (Abcam, Cambridge, UK), rabbit polyclonal EPO (Abcam, Cambridge, UK), and monoclonal mouse anti-mouse β-actin (Sigma, Saint Louis, MI, USA) were used at 1:400.

Techniques: Derivative Assay, Expressing, Western Blot

Journal: Antioxidants

Article Title: Intrarenal Arterial Transplantation of Dexmedetomidine Preconditioning Adipose Stem-Cell-Derived Microvesicles Confers Further Therapeutic Potential to Attenuate Renal Ischemia/Reperfusion Injury through miR-122-5p/Erythropoietin/Apoptosis Axis

doi: 10.3390/antiox11091702

Figure Lengend Snippet: Effect of H/R, miR-122-5p mimics (m), miR-122-5p inhibitor (I), or EPO on EPO, Bax, Bcl-2, C-Casp 3, and PARP expression in NRK-52E cells by Western blot ( A ). ( B ) Statistic data of EPO expression; ( C ) statistic data of Bax expression; ( D ) statistic data of Bcl-2 expression; ( E ) statistic data of C-Caspase 3 expression; and ( F ) Statistic data of PARP expression. Data are expressed as mean ± SEM (n = 3). C-Casp 3, cleaved caspase 3; EPO, erythropoietin; H/R, hypoxia/reoxygenation; PARP, poly-(ADP-ribose)-polymerase; SEM: standard error mean. * p < 0.05 compared with CON group. # p < 0.05 compared with H/R group.

Article Snippet: Antibodies raised against the monoclonal mouse anti-human PARP (Promega, Madison, WI, USA), polyclonal rabbit anti-rat caspase 3 (Chemicon, CA, USA), primary antibody Bcl-2 (Santa Cruz, Dallas, TX, USA), Bax (Abcam, Cambridge, UK), rabbit polyclonal EPO (Abcam, Cambridge, UK), and monoclonal mouse anti-mouse β-actin (Sigma, Saint Louis, MI, USA) were used at 1:400.

Techniques: Expressing, Western Blot

Journal: Antioxidants

Article Title: Intrarenal Arterial Transplantation of Dexmedetomidine Preconditioning Adipose Stem-Cell-Derived Microvesicles Confers Further Therapeutic Potential to Attenuate Renal Ischemia/Reperfusion Injury through miR-122-5p/Erythropoietin/Apoptosis Axis

doi: 10.3390/antiox11091702

Figure Lengend Snippet: The summary diagram. The summary diagram suggests that DEX-preconditioned ADSC-derived MVs can provide CD44 homing effect; ameliorate IR-induced renal hemodynamics depression; and reduce renal ROS level, tubular cell inflammation, apoptosis, tubular injury score, and renal dysfunction through downregulated miR-122-5p/Bax/PARP/apoptosis signaling and upregulated target-gene EPO expression in the post-IR kidney. DEX-preconditioned ADSC-derived MVs could be an important tool for utilization during renal IR injury. ADSC, adipose stem cells; AKI, acute kidney injury; DEX, dexmedetomidine; EPO, erythropoietin; MV, microvesicle; I/R, ischemia/reperfusion; PARP, poly-(ADP-ribose)-polymerase; ROS, reactive oxygen species.

Article Snippet: Antibodies raised against the monoclonal mouse anti-human PARP (Promega, Madison, WI, USA), polyclonal rabbit anti-rat caspase 3 (Chemicon, CA, USA), primary antibody Bcl-2 (Santa Cruz, Dallas, TX, USA), Bax (Abcam, Cambridge, UK), rabbit polyclonal EPO (Abcam, Cambridge, UK), and monoclonal mouse anti-mouse β-actin (Sigma, Saint Louis, MI, USA) were used at 1:400.

Techniques: Derivative Assay, Expressing